Effect of CDAP on myeloid-derived suppressor cells

Abstract

The aim of this study is to investigate the influence of Cistanche deserticola polysaccharide (CDAP) on myeloid-derived suppressor cells (MDSCs) within the tumor microenvironment. MTT assays were used to assess the viability of MDSCs, normal fibroblasts (NFs), and cancer associated fibroblasts (CAFs) following exposure to various concentrations of CDAP. Additionally, RT‒qPCR was used to measure the expression levels of VEGFA and TNF-γ in treated cells. Our results indicate that CDAP not only facilitates the growth of NFs while suppressing the occupancy of CAFs in the microenvironment but also diminishes the activity of MDSCs. Furthermore, CDAP-treated MDSCs exhibit upregulated TNF-γ expression alongside downregulated VEGFA expression. In conclusion, the traditional Chinese medicine polysaccharide CDAP exhibits a multifaceted capacity to enhance the tumor tissue microenvironment through its influence on various targets and pathways.